The cytotoxic effect of nasodren nasal spray (cyclamen europaeum extract) in L929 fibroblastic cell culture

Abstract

Na sod ren na sal spray (Cycla men eu ro pa e um ex tract), is used in so me co - un tri es as an al ter na ti ve the ra pe u tic agent for si nu si tis. The aim of this study was to ives ti ga te the pos sib le cyto to xic ef fects of the drug on cell morp ho logy and vi a bi lity in L929 mo u se fib - rob last cell cul tu re mo del. Ma te ri al and Met hods: Na sod ren so lu ti on was di lu ted to the dilutions of 1:25, 1:50, 1:100, 1:200, and 1:400 (w/v), and these dilutions were gro u ped from I to V, res pec ti vely. The con trol gro up was pre pa red by using sa li ne in a 1:1 ra ti o. L929 fib rob las tic cell cul tu re ma te ri al was in cu ba ted in 96-cell in cu ba ti on me di um. Fol lo wing a 12-ho ur in cu ba ti on pe ri od, the ma te ri al was ta ken in to fresh cul tu re me di um and was sto red for five days af ter tre at ment with va ri o us di lu ti ons of the test ma te ri al. Cell morp ho logy was ob ser ved under mic ros co pe on 1st, 2nd, 3rd, 4th, and 5th days. Cell vi a bi lity was eva lu a ted by 3-(4.5-di methy lthi a - zol-2-yl)-2,5-dip heny ltet ra zo li um bro mi de co lo ri met ric as say met hod on the sa me days. Re sults: Cells ma in ta i ned nor mal fib rob las tic sha pe and morp ho logy in Gro up V and the con trol gro up du ring the in cu ba ti on pe ri od. Nor mal ro und ring sha pe was apparently lost in Gro ups I-IV. Cell vi a bi lity dec re a sed in Gro ups I-IV, whe re as cell vi a bi lity in cre a sed in Gro up V and the con trol gro up. Conc lu si on: Re sults of this study in di ca te that hig her con cen tra ti ons of Cycla men eu ro - pa e um ha ve to xic ef fects on cell vi a bi lity in vit ro. The re is ne ed for in vi vo ani mal stu di es befo re sa fely pres cri bing this drug as the first li ne tre at ment of si nu si tis.