Application of vascular endothelial growth factor at different phases of intestinal ischemia/reperfusion: what are its effects on oxidative stress, inflammation and telomerase activity?
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CitationKorkmaz, A., Oyar, E., Yıldırım, Z., Pampal, A., Unlu, N., & Akbulut, H. (2020). Application of vascular endothelial growth factor at different phases of intestinal ischemia/reperfusion: What are its effects on oxidative stress, inflammation and telomerase activity? Advances in Clinical and Experimental Medicine, 29(12), 1417–1424. https://doi.org/10.17219/acem/126297
Background. Intestinal ischemic reperfusion injury (IRI) represents a great challenge in clinical practice, with high morbidity and mortality. Vascular endothelial growth factor (VEGF), asasignal protein, contributes tovasculogenesis and angiogenesis.Objectives. To evaluate the local effectiveness of VEGF following intestinal IRI and its relation with application time.Material and methods. Thirty Wistar albino rats were allocated to5 groups and underwent laparotomy. Thesuperior mesenteric arteries (SMA) were dissected in4 groups, while thecontrol group (GrC) underwent aresection ofsmall and large intestines. The VEGF group (Gr V) received VEGF following SMA dissection, with no further intervention, and theremaining 3 groups were subjected toischemia for 90min through occlusion ofSMA and reperfusion for 4h. Ischemic reperfusion group (Gr I/R) received no additional medication, while theremaining 2 groups received VEGF just before ischemia (Gr V+I/R) and during reperfusion (Gr I/R+V).Results. Both applications of VEGF caused decreases inplasma levels of interleukin6 (IL-6), tumor necrosis factor ? (TNF-?), intestinal malondialdehyde (MDA), oxidized glutathione, protein carbonyl levels, and increases inintestinal total glutathione and superoxide dismutase (SOD) levels. Telomerase activity, which disappeared for Gr I/R, was found to be elevated following both treatment groups. Similarly, the histopathological scores were found better for both treatment groups, but Gr V-I/R represented best outcomes.Conclusions. The findings of our study revealed that VEGF, applied either before ischemia or during reperfusion, iseffective onlocal damage following intestinal IRI. Byinterpreting thebiochemical analysis and histopathological findings, we conclude either treatment option to be considered according to the reason of intestinal IRI.